Cloning and assessment of mycobacterial promoters by using a plasmid shuttle vector
نویسندگان
چکیده
منابع مشابه
Cloning and assessment of mycobacterial promoters by using a plasmid shuttle vector.
We have constructed a promoter selection vector for mycobacteria to analyze the sequences involved in mycobacterial transcriptional regulation. The vector pSD7 contains extrachromosomal origins of replication from Escherichia coli as well as from Mycobacterium fortuitum and a kanamycin resistance gene for positive selection in mycobacteria. The promoterless chloramphenicol acetyltransferase (CA...
متن کاملassessment of the efficiency of s.p.g.c refineries using network dea
data envelopment analysis (dea) is a powerful tool for measuring relative efficiency of organizational units referred to as decision making units (dmus). in most cases dmus have network structures with internal linking activities. traditional dea models, however, consider dmus as black boxes with no regard to their linking activities and therefore do not provide decision makers with the reasons...
Molecular cloning of mycobacterial promoters in Escherichia coli.
Mycobacterium bovis BCG chromosomal DNA, digested with EcoR1 and HindIII, was used to construct a promoter library in Escherichia coli using the promoter probe plasmid pKO-1. DNA inserts of various sizes showed promoter activity judged by the level of galactokinase (galK) whose synthesis they activate (between 50 and 850 galactokinase units). No correlation between the length of the DNA insert ...
متن کاملNovel shuttle plasmid vehicles for Escherichia-Streptococcus transgeneric cloning.
A novel plasmid vector that is able to replicate both in Escherichia coli and in Streptococcus sanguis is described. This 9.2-kb plasmid, designated pVA856, carries Cmr, Tcr, and Emr determinants that are expressed in E. coli. Only the Emr determinant is expressed in S. sanguis. Both the Cmr and the Tcr of pVA856 may be insertionally inactivated. This plasmid affords several different cleavage-...
متن کاملan investigation into translation of cultural concepts by beginner and advance student using think – aloud protocols
this research aims at answering the questions about translation problems and strategies applied by translators when translating cultural concepts. in order to address this issue, qualitative and quantitative study were conducted on two groups of subjects at imam reza international university of mashhad. these two groups were assigned as beginner and advanced translation students (10 students). ...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1993
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.175.16.5186-5192.1993